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Calculate median CV for phosphorylation sites across abundance tiers: High abundance (e.g., p-AKT S473) Medium abundance (e.g., p-ERK T202/Y204) Low abundance (e.g., p-STAT1 Y701) Key Statistical Power Parameters: Effect Size (d): Convert fold change via d = (M₁-M₂)/SD (e.g., 1.5-fold ≈ *d*=0.5-0.7) Significance Threshold (α): Default = 0.05 Statistical Power (1-β): Recommended ≥0.8 Test Design: Two-sample t-test (two.sided) Sample Size Interpretation: For *d*=0.6 (≈1.5-fold change): 12 replicates/group (calculated *n*=11.3 → rounded up) For *d*=0.8 (≈1.8-fold change): 7 replicates/group Cost-Constrained Alternatives: Prioritize Top 50 most significantly altered sites (reducing multiple testing burden) Adopt paired-sample design (pre- vs
Mature Skin
Program:12 Week Regenerative Renewal
MS 100 series (MS 151, MS 161): Special wax treatment, does not affect transparency and whiteness, with anti-sedimentation, and surface smoothness
