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Salt molecules in the buffer dehydrate the hydrophobic surfaces of proteins, thereby increasing their attraction to the stationary phase
festive_all-rounder
Size:25G
the reagents required are as follows: UV sterilized 2X-deionized water or sterile water for irrigation Braun R5000-01 Hylumed Medical - Sodium Hyaluronate, High Molecular Weight HA Genzyme Advanced Biomaterials 4876 Hyaluronidase Reference Standard USP 31200 Potassium Acetate, Granular, USP, ACS JTBaker 2914-01 Acetic Acid, Glacial, 99+ % Sigma A-6283 Sodium Phosphate Monobasic Monohydrate, USP Granular Mallinkrodt 7774 Sodium Phosphate Dibasic Anhydrous, USP Mallinkrodt 7771 Sodium Chloride, Crystals, GR, ACS EMScience SX0420-5 Gelatin Hydrolysate Enzymatic Sigma G-0262 Horse Serum, Donor Herd, cell culture tested, Hybridoma culture tested, USA Origin Sigma H-1270 Human Se Serum Stock Solution -Dilute 1 volume of Horse Serum with 9 volumes of Acetate Buffer Solution